Perspectives

CryoLetters launched its series of PERSPECTIVES articles in the middle of 2020. They aim to provide an overview of recent developments in cryobiology and cryobiotechnology that will appeal to a wide readership.

These mini-reviews are available as Open Access PDF articles, at no cost to the authors. Please submit your suggestions to editor@cryoletters.org.

2025 (Volume 46)

PERSPECTIVE: Production and cryopreservation of 3D cultures

Nataliia Moisieieva, Olga Gorina, Anton Moisieiev and Olga Prokopiuk

Volume 46 (1), 1-13 (2025)

doi.org/10.54680/fr25110110112

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PERSPECTIVE: Strategies used by organisms to survive very cold climates – student‘s guide

S. P. Chesterfield, H. V. Joyce, T. D. Fooks and P. W. Wilson

Volume 46 (2), 67-73 (2025)

doi.org/10.54680/fr25210110112

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PERSPECTIVE: Challenges in bird cryopreservation

Juan Pérez-Rivero

Volume 46 (2), 74-81 (2025)

doi.org/10.54680/fr25210110312

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PERSPECTIVE: Challenges and recent progress on the use of cryobiotechnology for conserving Brazilian native plants

Georgia Pacheco, Marcela Gomes Vianna-Almeida, Renata de Oliveira Garcia and Elisabeth Mansur

Volume 46 (3), 143-163 (2025)

doi.org/10.54680/fr25310110112

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2024 (Volume 45)

PERSPECTIVE: The comet assay as a method for assessing DNA damage in cryopreserved samples

Beata P. Plitta-Michalak, Alice Ramos, Dominika Stępień, Magdalena Trusiak and Marcin Michalak

Volume 45 (1), 1-15 (2024)

doi.org/10.54680/fr24110110112

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PERSPECTIVE: Targeted development and optimization of small-molecule ice recrystallization inhibitors (IRIs) for the cryopreservation of biological systems

Leah E. McMunn, Ellyssa M. Walsh and Robert N. Ben

Volume 45 (2), 69-87 (2024)

doi.org/10.54680/fr24210110112

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PERSPECTIVE: The cold chain delivery of organs for transplantation: from research laboratories and individual enthusiasts to pan-global networks in 50 years

Barry J. Fuller & Colin C. Green

Volume 45 (3), 139-148 (2024)

doi.org/10.54680/fr24310110112

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PERSPECTIVE: Semen additives for improving frozen-thawed buffalo and cattle semen – a review

Sirivati Venkatesh, K. Murugavel, H. Hemalatha, S. Kantharaj and G. Shalini

Volume 45 (4), 194-211 (2024)

doi.org/10.54680/fr24410110112

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PERSPECTIVE: Cryosurgery process applications: a mathematical review

Lalhmingsangi Famhawite, Sonam Tanwar and Pooja Raj Verma

Volume 45 (5), 269-278 (2024)

doi.org/10.54680/fr24510110112

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PERSPECTIVE: Transcriptomics of cryopreserved cells

Yelda Özden Çiftçi and Ergun Kaya

Volume 45 (6), 329-339 (2024)

doi.org/10.54680/fr24610110112

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2023 (Volume 44)

PERSPECTIVE: Unifying principles of cryopreservation protocols for new plant materials based on alternative cryoprotective agents (CPAs) and a systematic approach

Haeng-Hoon Kim and Elena Popova

Volume 44 (1), 1-12 (2023)

doi.org/10.54680/fr23110110112

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PERSPECTIVE: Cryopreservation of organoids

Olena Rogulska, Jarmila Havelkova and Yuriy Petrenko

Volume 44 (2), 65-75 (2023)

doi.org/10.54680/fr23210110112

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PERSPECTIVE: The role of cryopreservation techniques in manufacturing, transport, and storage of CAR-T therapy products

Miroslava Jandová, Glyn Nigel Stacey, Miriam Lánská, Jiří Gregor, Petra Rozsívalová, Lenka Beková, Zuzana Woidigová Ducháčová, David Belada, Jakub Radocha, Pavel Měřička and Barry Fuller

Volume 44 (3), 123-133 (2023)

doi.org/10.54680/fr23310110112

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PERSPECTIVE: Cryobiology and fertility preservation: a perspective on past, current and future studies

Naeimeh Sadat Abtahi, Zeinab Ghezelayagh, Iran Nemati, Farideh Eivazkhani, Parvaneh Farzaneh, Abdolhossein Shahverdi, Gholam Reza Goudarzi, Abdurrahim Pedram, Elham Amirchaghmaghi, Mojtaba Rezazadeh Valojerdi, Sherman Silber and Rouhollah Fathi

Volume 44 (4), 185-196 (2023)

doi.org/10.54680/fr23410110112

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PERSPECTIVE: Potential and reality of cryopreserving somatic cells of wild felids for conservation

Luanna Lorenna Vieira Rodrigues and Alexsandra Fernandes Pereira

Volume 44 (5), 249-257 (2023)

doi.org/10.54680/fr23510110112

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PERSPECTIVE: The technology in cryotechnology

Janina Hagedorn, Ghiath Alkurdi, Sven-Alexander Barker, Ricarda Brunotte, Tarek Deeb, Oleksandra Hubenia, Diaa Khayyat, Sara Leal-Marin, Tim Rittinghaus and Birgit Glasmacher

Volume 44 (6), 314-326 (2023)

doi.org/10.54680/fr23610110112

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2022 (Volume 43)

PERSPECTIVE: Temperature-dependent density and thermal expansion of cryoprotective agents

Prem K. Solanki and Yoed Rabin

Volume 43 (1), 1-9 (2022)

doi.org/10.54680/fr22110110112

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PERSPECTIVE: Systemic and local hypothermia in the context of cell regeneration

Basheer Abdullah Marzoog

Volume 43 (2), 66-73 (2022)

doi.org/10.54680/fr22210110112

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PERSPECTIVE: Recent advancements in vitrification cryodevices for gamete and gonadal tissue

Masrat-Un-Nisa, Asloob Ahmad Malik, Khursheed Ahmad Sofi, Arjuma Khatun and Nahida Yousuf

Volume 43 (3), 129-139 (2022)

doi.org/10.54680/fr22310110112

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PERSPECTIVE: A state of the art review of isochoric cryopreservation and cryoprotectant

George-Andrei Beşchea, Ştefan-Ioan Câmpean, Maria-Bianca Tăbăcaru, Beatrice-Georgiana Vuţoiu, Alexandru Şerban and Gabriel Năstase

Volume 43 (4), 189-199 (2022)

doi.org/10.54680/fr22410110112

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PERSPECTIVE: Hypothermic organ perfusion in the 2020s: mixing the benefits of low temperatures and dynamic flow outside the body

Daria Cherkashina, Oleksandr Petrenko and Barry Fuller

Volume 43 (5), 255-263 (2022)

doi.org/10.54680/fr22510110112

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PERSPECTIVE: A guide to successful mL to L scale vitrification and rewarming

Lakshya Gangwar, Shaunak S. Phatak, Michael Etheridge and John C. Bischof

Volume 43 (6), 303-315 (2022)

doi.org/10.54680/fr22610110112

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2021 (Volume 42) Please note that abstracts from this volume and earlier are in the Abstracts Archive

PERSPECTIVE: Encapsulation nanotechnology in sperm cryopreservation: systems preparation methods and antioxidants enhanced delivery

Lamia Taouzinet, Sofiane Fatmi, Malika Lahiani-Skiba, Mohamed Skiba and Mokrane Iguer-Ouada

Abstracts Archive: Volume 42 (1), 1-12 (2021)

doi.org/10.54680/fr21110110112

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PERSPECTIVE: Rooster spermatozoa cryopreservation and quality assessment

Andrea Svoradová, Lenka Kuželová, Jaromír Vašíček, Andrej Baláži, Lucia Olexiková, Alexander Makarevich and Peter Chrenek

Abstracts Archive: Volume 42 (2), 59-66 (2021)

doi.org/10.54680/fr21210110112

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PERSPECTIVE: Cryopreservation of human oocytes and the 'carryover' effect on early embryo development

Qi-Peng Jia and Wendell Q. Sun

Abstracts Archive: Volume 42 (3), 120-128 (2021)

doi.org/10.54680/fr21310110112

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PERSPECTIVE: Freezing protocols for the cryopreservation of immature testicular tissue – a systematic review

Bríd Ní Dhonnabhàin and Natalie Getreu

Abstracts Archive: Volume 42 (4), 188-201 (2021)

doi.org/10.54680/fr21410110112

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PERSPECTIVE: Oocyte and embryo preservation in wild animals: an update

G R Bhat and K A Sofi

Abstracts Archive: Volume 42 (5), 251-260 (2021)

doi.org/10.54680/fr21510110112

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PERSPECTIVE: Genome resource banking for wildlife conservation: promises and caveats

William V. Holt and Pierre Comizzoli

Abstracts Archive: Volume 42 (6), 309-320 (2021)

doi.org/10.54680/fr21610110112

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2020 (Volume 41) Please note that abstracts from this volume and earlier are in the Abstracts Archive

PERSPECTIVE: Pollen cryopreservation for plant breeding and genetic resources conservation

Naiana Barbosa Dinato, Izulmé Rita Imaculada Santos, Bianca Baccili Zanotto Vigna, Ailton Ferreira de Paula and Alessandra Pereira Fávero

Abstracts Archive: Volume 41 (3), 115-127 (2020)

doi.org/10.54680/fr20310110112

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PERSPECTIVE: Critical cooling and warming rates as a function of CPA concentration

Zonghu Han and John C. Bischof

Abstracts Archive: Volume 41 (4), 185-193 (2020)

doi.org/10.54680/fr20410110112

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PERSPECTIVE: Possible protective mechanisms of coenzyme Q10 action on spermatozoa during cryopreservation or cooled-stored condition

Michael Osei Appiah, Bismark Asante-Badu, Jing Zhao, Hongyu Liu, Jun Wang and Wenfa Lu

Abstracts Archive: Volume 41 (5), 246-256 (2020)

doi.org/10.54680/fr20510110112

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PERSPECTIVE: Nanoparticle-mediated delivery of cryoprotectants for cryopreservation

Samantha Stewart, Alyssa Arminan and Xiaoming He

Abstracts Archive: Volume 41 (6), 308-316 (2020)

doi.org/10.54680/fr20610110112

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