Perspectives

CryoLetters launched its series of PERSPECTIVES articles in the middle of 2020. They aim to provide an overview of recent developments in cryobiology and cryobiotechnology that will appeal to a wide readership.

These mini-reviews are available as Open Access PDF articles, at no cost to the authors. Please submit your suggestions to editor@cryoletters.org.

2025 (Volume 46)

PERSPECTIVE: Production and cryopreservation of 3D cultures

Nataliia Moisieieva, Olga Gorina, Anton Moisieiev and Olga Prokopiuk

Volume 46 (1), 1-13 (2025)

doi.org/10.54680/fr25110110112

PDF

PERSPECTIVE: Strategies used by organisms to survive very cold climates – student‘s guide

S. P. Chesterfield, H. V. Joyce, T. D. Fooks and P. W. Wilson

Volume 46 (2), 67-73 (2025)

doi.org/10.54680/fr25210110112

PDF

PERSPECTIVE: Challenges in bird cryopreservation

Juan Pérez-Rivero

Volume 46 (2), 74-81 (2025)

doi.org/10.54680/fr25210110312

PDF

PERSPECTIVE: Challenges and recent progress on the use of cryobiotechnology for conserving Brazilian native plants

Georgia Pacheco, Marcela Gomes Vianna-Almeida, Renata de Oliveira Garcia and Elisabeth Mansur

Volume 46 (3), 143-163 (2025)

doi.org/10.54680/fr25310110112

PDF

PERSPECTIVE: Advancements in cryopreservation techniques for human gametes and embryos: novel cryoprotectants and their influence on fertility preservation

A. S. Vickram, C. Prasanth, S. Bharath, D. Jenila Rani, C. M. Mathan Muthu and B. Bhavani Sowndharya

Volume 46 (4), 213-230 (2025)

doi.org/10.54680/fr25410110112

PDF

2024 (Volume 45)

PERSPECTIVE: The comet assay as a method for assessing DNA damage in cryopreserved samples

Beata P. Plitta-Michalak, Alice Ramos, Dominika Stępień, Magdalena Trusiak and Marcin Michalak

Volume 45 (1), 1-15 (2024)

doi.org/10.54680/fr24110110112

PDF

PERSPECTIVE: Targeted development and optimization of small-molecule ice recrystallization inhibitors (IRIs) for the cryopreservation of biological systems

Leah E. McMunn, Ellyssa M. Walsh and Robert N. Ben

Volume 45 (2), 69-87 (2024)

doi.org/10.54680/fr24210110112

PDF

PERSPECTIVE: The cold chain delivery of organs for transplantation: from research laboratories and individual enthusiasts to pan-global networks in 50 years

Barry J. Fuller & Colin C. Green

Volume 45 (3), 139-148 (2024)

doi.org/10.54680/fr24310110112

PDF

PERSPECTIVE: Semen additives for improving frozen-thawed buffalo and cattle semen – a review

Sirivati Venkatesh, K. Murugavel, H. Hemalatha, S. Kantharaj and G. Shalini

Volume 45 (4), 194-211 (2024)

doi.org/10.54680/fr24410110112

PDF

PERSPECTIVE: Cryosurgery process applications: a mathematical review

Lalhmingsangi Famhawite, Sonam Tanwar and Pooja Raj Verma

Volume 45 (5), 269-278 (2024)

doi.org/10.54680/fr24510110112

PDF

PERSPECTIVE: Transcriptomics of cryopreserved cells

Yelda Özden Çiftçi and Ergun Kaya

Volume 45 (6), 329-339 (2024)

doi.org/10.54680/fr24610110112

PDF

2023 (Volume 44)

PERSPECTIVE: Unifying principles of cryopreservation protocols for new plant materials based on alternative cryoprotective agents (CPAs) and a systematic approach

Haeng-Hoon Kim and Elena Popova

Volume 44 (1), 1-12 (2023)

doi.org/10.54680/fr23110110112

PDF

PERSPECTIVE: Cryopreservation of organoids

Olena Rogulska, Jarmila Havelkova and Yuriy Petrenko

Volume 44 (2), 65-75 (2023)

doi.org/10.54680/fr23210110112

PDF

PERSPECTIVE: The role of cryopreservation techniques in manufacturing, transport, and storage of CAR-T therapy products

Miroslava Jandová, Glyn Nigel Stacey, Miriam Lánská, Jiří Gregor, Petra Rozsívalová, Lenka Beková, Zuzana Woidigová Ducháčová, David Belada, Jakub Radocha, Pavel Měřička and Barry Fuller

Volume 44 (3), 123-133 (2023)

doi.org/10.54680/fr23310110112

PDF

PERSPECTIVE: Cryobiology and fertility preservation: a perspective on past, current and future studies

Naeimeh Sadat Abtahi, Zeinab Ghezelayagh, Iran Nemati, Farideh Eivazkhani, Parvaneh Farzaneh, Abdolhossein Shahverdi, Gholam Reza Goudarzi, Abdurrahim Pedram, Elham Amirchaghmaghi, Mojtaba Rezazadeh Valojerdi, Sherman Silber and Rouhollah Fathi

Volume 44 (4), 185-196 (2023)

doi.org/10.54680/fr23410110112

PDF

PERSPECTIVE: Potential and reality of cryopreserving somatic cells of wild felids for conservation

Luanna Lorenna Vieira Rodrigues and Alexsandra Fernandes Pereira

Volume 44 (5), 249-257 (2023)

doi.org/10.54680/fr23510110112

PDF

PERSPECTIVE: The technology in cryotechnology

Janina Hagedorn, Ghiath Alkurdi, Sven-Alexander Barker, Ricarda Brunotte, Tarek Deeb, Oleksandra Hubenia, Diaa Khayyat, Sara Leal-Marin, Tim Rittinghaus and Birgit Glasmacher

Volume 44 (6), 314-326 (2023)

doi.org/10.54680/fr23610110112

PDF

2022 (Volume 43)

PERSPECTIVE: Temperature-dependent density and thermal expansion of cryoprotective agents

Prem K. Solanki and Yoed Rabin

Volume 43 (1), 1-9 (2022)

doi.org/10.54680/fr22110110112

PDF

PERSPECTIVE: Systemic and local hypothermia in the context of cell regeneration

Basheer Abdullah Marzoog

Volume 43 (2), 66-73 (2022)

doi.org/10.54680/fr22210110112

PDF

PERSPECTIVE: Recent advancements in vitrification cryodevices for gamete and gonadal tissue

Masrat-Un-Nisa, Asloob Ahmad Malik, Khursheed Ahmad Sofi, Arjuma Khatun and Nahida Yousuf

Volume 43 (3), 129-139 (2022)

doi.org/10.54680/fr22310110112

PDF

PERSPECTIVE: A state of the art review of isochoric cryopreservation and cryoprotectant

George-Andrei Beşchea, Ştefan-Ioan Câmpean, Maria-Bianca Tăbăcaru, Beatrice-Georgiana Vuţoiu, Alexandru Şerban and Gabriel Năstase

Volume 43 (4), 189-199 (2022)

doi.org/10.54680/fr22410110112

PDF

PERSPECTIVE: Hypothermic organ perfusion in the 2020s: mixing the benefits of low temperatures and dynamic flow outside the body

Daria Cherkashina, Oleksandr Petrenko and Barry Fuller

Volume 43 (5), 255-263 (2022)

doi.org/10.54680/fr22510110112

PDF

PERSPECTIVE: A guide to successful mL to L scale vitrification and rewarming

Lakshya Gangwar, Shaunak S. Phatak, Michael Etheridge and John C. Bischof

Volume 43 (6), 303-315 (2022)

doi.org/10.54680/fr22610110112

PDF

2021 (Volume 42) Please note that abstracts from this volume and earlier are in the Abstracts Archive

PERSPECTIVE: Encapsulation nanotechnology in sperm cryopreservation: systems preparation methods and antioxidants enhanced delivery

Lamia Taouzinet, Sofiane Fatmi, Malika Lahiani-Skiba, Mohamed Skiba and Mokrane Iguer-Ouada

Abstracts Archive: Volume 42 (1), 1-12 (2021)

doi.org/10.54680/fr21110110112

PDF

PERSPECTIVE: Rooster spermatozoa cryopreservation and quality assessment

Andrea Svoradová, Lenka Kuželová, Jaromír Vašíček, Andrej Baláži, Lucia Olexiková, Alexander Makarevich and Peter Chrenek

Abstracts Archive: Volume 42 (2), 59-66 (2021)

doi.org/10.54680/fr21210110112

PDF

PERSPECTIVE: Cryopreservation of human oocytes and the 'carryover' effect on early embryo development

Qi-Peng Jia and Wendell Q. Sun

Abstracts Archive: Volume 42 (3), 120-128 (2021)

doi.org/10.54680/fr21310110112

PDF

PERSPECTIVE: Freezing protocols for the cryopreservation of immature testicular tissue – a systematic review

Bríd Ní Dhonnabhàin and Natalie Getreu

Abstracts Archive: Volume 42 (4), 188-201 (2021)

doi.org/10.54680/fr21410110112

PDF

PERSPECTIVE: Oocyte and embryo preservation in wild animals: an update

G R Bhat and K A Sofi

Abstracts Archive: Volume 42 (5), 251-260 (2021)

doi.org/10.54680/fr21510110112

PDF

PERSPECTIVE: Genome resource banking for wildlife conservation: promises and caveats

William V. Holt and Pierre Comizzoli

Abstracts Archive: Volume 42 (6), 309-320 (2021)

doi.org/10.54680/fr21610110112

PDF

2020 (Volume 41) Please note that abstracts from this volume and earlier are in the Abstracts Archive

PERSPECTIVE: Pollen cryopreservation for plant breeding and genetic resources conservation

Naiana Barbosa Dinato, Izulmé Rita Imaculada Santos, Bianca Baccili Zanotto Vigna, Ailton Ferreira de Paula and Alessandra Pereira Fávero

Abstracts Archive: Volume 41 (3), 115-127 (2020)

doi.org/10.54680/fr20310110112

PDF

PERSPECTIVE: Critical cooling and warming rates as a function of CPA concentration

Zonghu Han and John C. Bischof

Abstracts Archive: Volume 41 (4), 185-193 (2020)

doi.org/10.54680/fr20410110112

PDF

PERSPECTIVE: Possible protective mechanisms of coenzyme Q10 action on spermatozoa during cryopreservation or cooled-stored condition

Michael Osei Appiah, Bismark Asante-Badu, Jing Zhao, Hongyu Liu, Jun Wang and Wenfa Lu

Abstracts Archive: Volume 41 (5), 246-256 (2020)

doi.org/10.54680/fr20510110112

PDF

PERSPECTIVE: Nanoparticle-mediated delivery of cryoprotectants for cryopreservation

Samantha Stewart, Alyssa Arminan and Xiaoming He

Abstracts Archive: Volume 41 (6), 308-316 (2020)

doi.org/10.54680/fr20610110112

PDF